RESUMO
Gainoffunction (GOF) mutations in the TP53 gene lead to acquisition of new functions by the mutated tumor suppressor p53 protein. A number of the overrepresented 'hot spot' mutations, including the ones in codons 175, 248 or 273, convey GOF phenotypes. Such phenotypes may include resistance to chemotherapeutics or changes in motility and invasiveness. Whereas the prevalent notion is that the acquisition of the p53 GOF phenotype translates into poorer prognosis for the patient, the analysis of a human somatic p53 mutations dataset demonstrated earlier tumor onset, but decreased frequency and altered location of metastases in patients with the p53R248Q allele. Therefore, the GOF activities of p53R248Q and p53D281G were analyzed in triple negative breast cancer MDAMB231 and lung adenocarcinoma H1299 cell lines with regard to invasive and metastatic traits. The expression of p53D281G increased the motility and invasiveness of the lung cancer cells, but not those of the breast cancer cells. In contrast, the expression of p53R248Q decreased the motility and invasiveness of the breast and lung cancer cells in a p53 transactivationdependent manner. The intravenous xenotransplantation of MDAMB231 cells expressing p53R248Q into zebrafish embryos resulted in an alteration of the distribution of cancer cells in the body of the fish. In p53R248Qexpressing H1299 cells a decrease in the expression of TCF8/ZEB1 and Ncadherin was observed, suggesting partial mesenchymaltoepithelial transition. In the two cell lines expressing p53R248Q a decrease was noted in the expression of myosin light chain 2, a protein involved in actomyosinbased motility. To the best of our knowledge, the present study is one of only few reports demonstrating the mutated p53 GOF activity resulting in a decrease of a malignant trait in human cancer.